【代表性论文】:
[1] Jian Wang†, Rui Hao†, Tianfang Jiang†, Xuanxuan Guo, Fei Zhou, Limei Cao, Fengjuan Gao, Guangming Wang, Juan Wang, Ke Ning, Chunlong Zhong*, Xu Chen*, Ying Huang*, Jun Xu*, and Shane Gao*,Rebuilding Hippocampus Neural Circuit with hADSC-derived Neuron Cells for Treating Ischemic Stroke, Cell & Bioscience, 2022 (Pre-print) (IF=7.13) 人源脂肪干细胞通过重建海马神经环路治疗缺血性脑卒中。
[2] Xuanxuan Guo1†, Chenqi Lu2†, Yaqun Lu1†, Simeng Zhao3, Jian Wang1, Juan Wang4, Fei Zhou5, Qiang Li6*, Chunlong Zhong1*, Shane Gao1*,Human adipose-derived stem cells (hADSCs) proceed through the neural stem cell stage before committing their neuron or motor neuron cell fate, iScience, 2022 (Pre-print) (IF=5.8) 人源脂肪干细胞干细胞在向神经细胞或运动神经元分化命运开启前需要经历神经干细胞阶段。
[3] Shane Gao, XuanXuan Guo, Simeng Zhao, Yinpeng Jin, Fei Zhou, Ping Yuan, Limei Cao, Jian Wang, Yue Qiu, Chenxi Sun, Zhanrong Kang, Guisheng Zhong*, Liming Cheng*, Hongwen Zhu*, Zhengliang Gao*, Xu Chen*, Jun Xu*, Differentiation of human adipose-derived stem cells into neuron/motoneuron-like cells for cell replacement therapy of spinal cord injury, Cell Death & Disease, 2019 Aug; 10(8): 597(IF=8.469).人脂肪干细胞分化为神经元/运动神经元样细胞用于脊髓损伤、细胞死亡和疾病的细胞替代治疗。
[4] Li Q†, Gao S†, Kang Z, Zhang M, Zhao X, Zhai Y, Huang J, Yang G-Y, Sun W* and Wang J*, Rapamycin Enhances Mitophagy and Attenuates Apoptosis After Spinal Ischemia-Reperfusion Injury. Front. Neurosci. 2018,12:865.(IF=4.677).雷帕霉素增强脊髓缺血-再灌注损伤后线粒体吞噬和减少细胞凋亡。
[5] Jin Y, Wang J, Li H, Gao S, Shi R, Yang D, Wang X, Wang X, Zhu L, Wang X, Chen C, Ning K, Gao Z*, Xu J*, Fu Q*. Extracellular Vesicle Secreted by Human Adipose-Derived Stem cells (hASCs) Improve Survival Rate of Rats with Acute Liver Failure by Releasing lncRNA H19. EBioMedicine, 2018, Aug 34:231-242 (IF=8.143).人脂肪源性干细胞(hADSCs)分泌的胞外囊泡通过释放lncRNA H19提高急性肝功能衰竭大鼠的存活率
[6] Tianfang Jiang, Gen Li, Jun Xu, Shane Gao*, Xu Chen*. The Challenge of the Pathogenesis of Parkinson's Disease: Is Autoimmunity the Culprit? Front Immunol. 2018, 9: 2047.(IF=7.561).帕金森氏症发病机制的挑战:自身免疫是罪魁祸首吗?
[7] Rongjie Ma, Min Wang, Shane Gao, Liang Zhu, Liming Yu, Daiyu Hu, Luying Zhu,Wei Huang, Weihua Zhang, Jiajia Deng, Jie Pan, Hua He, Zhengliang Gao, Jun Xu*, Xinxin Han*. miR-29a Promotes the Neurite Outgrowth of Rat Neural Stem Cells by Targeting Extracellular Matrix to Repair Brain Injury, Stem Cells Dev. 2019 Dec; 12: (IF=3.272). miR-29a通过靶向细胞外基质促进大鼠神经干细胞神经突生长修复脑损伤。
[8] Wang G, Wang F, Ren J, Qiu Y, Zhang W, Gao S, Yang D, Wang Z, Liang A, Gao Z, Xu J. SIRT1 Involved in the Regulation of Alternative Splicing Affects the DNA Damage Response in Neural Stem Cells. Cell Physiol Biochem, 2018, 48(2):657-669. (IF=5.5). SIRT1参与调节选择性剪接对神经干细胞DNA损伤反应的影响
[9] Gao FJ†, Gao S†, Chen X, Sun J, Wang JY, Anti-inflammatory effect of stem cells in the treatment of ischemic stroke, Chinese Journal of Tissue Engineering Research, 2017, 21(25):4088-4093. (IF=1.272) 干细胞在治疗缺血性脑卒中中的抗炎效果。
[10] Wang J, Li JX, Yang Jian, Zhang Liang LG, Gao S, Jiao F, Yi ML, Xu J. MicroRNA-138-5p regulates neural stem cell proliferation and differentiation in vitro by targeting TRIP6 expression. Mol Med Rep. 2017, 16(5): 7261–7266. (IF=2.952) MicroRNA-138-5p 通过TRIP 6调控神经干细胞的增殖和分化。
[11] Zhou F †, Gao S †, Wang L †, Sun C, Chen L, Yuan P, Zhao H, Yi Y, Qin Y, Dong Z, Cao L, Ren H, Zhu L, Li Q, Lu B, Liang A, Xu GT, Zhu H, Gao Z, Ma J*, Xu J*, Chen X*. Human adipose-derived stem cells partially rescue the stroke syndromes by promoting spatial learning and memory in mouse middle cerebral artery occlusion model. Stem Cell Res Ther. 2015, 6(1):92-104.(IF=6.832)人源脂肪干细胞通过提高中风小鼠的学习记忆能力改善其中风症状。
[12] Gao S†, Zhao P†, Lin C, Sun Y, Wang Y, Zhou Z, Yang D, Wang X, Xu H, Zhou F, Cao L, Zhou W, Ning K, Chen X*, Xu J*. Differentiation of human adipose-derived stem cells into neuron-like cells which are compatible with photocurable three-dimensional scaffolds. Tissue Eng Part A. 2014, 20(7-8):1271-1284. (IF=3.845)人源脂肪干细胞通过与3D 光敏感生物材料结合实现神经分化。
[13] Zhou F†, Gao S†, Sun C, Cao L, Ren H, Li Q, Wang G, Dong C, Zhu L, Yang D, Wang X, Xu J*, Chen X*. Adipose-derived stem cells in stroke treatment: translational possibility and mechanism. Chin Med J (Engl). 2014, 127(20):3657-3663. (IF=2.628)脂肪干细胞治疗中风:转化可能性和机理。
[14] Shi S†, Gao S†, Cao T, Liu J, Gao X, Hao J, Lv C, Huang H, Xu J*, Yao T*. Targeting Human Telomeric G-Quadruplex DNA and Inhibition of Telomerase Activity With [(dmb)2Ru(obip)Ru(dmb)2]4+, PLos One, 2013, 8(12).e84419. (IF=3.240) [(dmb)2Ru(obip)Ru(dmb)2]4+ 通过结合端粒末端G-Quadruplex DNA结构抑制端粒酶活性。
[15] Yang DJ, Wang XL, Ismail A, Ashman CJ, Valori CF, Wang G, Gao S, Higginbottom A, Ince PG, Azzouz M, Xu J*, Shaw PJ*, Ning K*, PTEN regulates AMPA receptor-mediated cell viability in iPS-derived motor neurons,Cell Death & Disease, 2014, 5(e1096). (IF=8.469)PTEN 通过调节AMPA调控iPS分化的运动神经元。
[16] Dong CM, Wang XL, Wang GM, Zhang WJ, Zhu L, Gao S, Yang DJ, Qin Y, Liang QJ, Chen YL, Deng HT, Ning K, Liang AB, Gao ZL, Xu J. A stress-induced cellular aging model with postnatal neural stem cells, Cell Death & Disease, 2014, 5(e1116).(IF=8.469) 应激诱导的产后神经干细胞细胞老化模型。
[17] Shane Gao †*, Juan Wang †, Zeyang Wu, Ping Yuan, Fengjuan Gao, Fei Zhou, Limei Cao, Xu Chen, Hongwen Zhu, Jun Xu, Do the Stem Cells Really Work with Autism Spectrum Disorders Associated with Neuro-Immune Interaction? Autism Open Access, 2015, 5(3):151-157. 干细胞真的能治疗与神经免疫相互作用相关的自闭症谱系障碍吗? 【专利获奖】
【1】徐俊,高山峨,人源脂肪干细胞来源的神经元样细胞及其制备方法和应用,中国, ZL 2013 1 0161558.3 发明专利,申请人:东方医院。(授权日期:2018-7-17)
Jun Xu, Shane Gao, Preparation and Application of Neuron-like Cells derived from Human Adipose stem cells, China, ZL 2013 1 0161558.3 Invention patent, applicant: Dongfang Hospital. (Authorization Date: July 17, 2018).
【2】徐俊,高山峨,朱红文,脂肪干细胞来源的运动神经元样细胞及其制备方法和应用,中国,ZL 2014 1 0160948. 3发明专利,申请人:东方医院,天津医院。(授权日期:2020-6-26)
Jun Xu, Shane Gao, Hongwen Zhu, Preparation and Application of Motor neuronlike Cells derived from Adipose stem cells, China, ZL 2014 1 0160948.3 Invention patent, applicant: Dongfang Hospital, Tianjin Hospital. (Authorized Date: 2020-6-26).
【3】陈旭,徐俊,高山峨,“人源脂肪干细胞体外神经分化及改善缺血性脑卒中小鼠行为学的研究”,上海医学科技奖三等奖,第三发明人,2015.6
Xu Chen, Jun Xu, Shan 'e Gao, "Neural differentiation of human adipocyte stem cells in vitro and improvement of behavior in mice with ischemic stroke", Third prize of Shanghai Medical Science and Technology Award, third invention, 2015.6.
【4】高山峨,人源脂肪干细胞治疗小鼠脊髓损伤的研究,国家级实验教学示范中心联席会、中国高等医学教育学会基础医学教育分会和教育部高等学校医药学科基础医学专业与课程教学指导委员会,2014年第三届全国大学生基础医学创新论坛暨实验设计大赛,优胜奖,2014。(周志,李涵,唐丹粒,高山峨*)奖励。
Shane Gao,Treatment of spinal cord injury in mice with human adipose stem cells,National Demonstration Center of Experimental Teaching Joint meeting,Basic Medical Education Branch of China Association of Higher Medical Education and Basic Medical Specialty and Curriculum Teaching Steering Committee of Medical Discipline of Higher Education of Ministry of Education,2014 The third National College Students Basic Medical Innovation Forum and Experimental Design Competition,Winning reward, 2014. (Zhi Zhou, Han Li, Danli Tang, Shane Gao*)Award.
【5】高山峨,用实时定量PCR来检测脂肪干细胞干性相关基因的表达,同济大学,同济大学第六期大学生创新实践训练计划(SITP-Ⅵ),一等奖,2012。(孙玉玺, 王艺蕾,周志翀,高山峨)。 奖励
Shane Gao, Detect the expression of dryness related genes in adipose stem cells by using the real-time quantitative PCR, Tongji University, The sixth innovation Practice Training Program for College students of Tongji University(SITP-Ⅵ),First prize,2012.(Yuxi Sun, Yilei Wang, Zhichong Zhou,Shane Gao). Award. 【论著、专家共识及会议论文】:
[1] 中国抗癌协会肿瘤与微生态专业委员会.肿瘤与微生态领域:高山峨(同济大学医学院)肠道微生态与造血干细胞移植相关性中国专家共识[J].国际肿瘤学杂志,2021,48(03):129-135.
Cancer and Microecology Committee of Chinese Anti-Cancer Association, Oncology and microecology, Shane Gao (Medical College of Tongji University) Chinese expert consensus on the correlation between intestinal microecology and hematopoietic stem cell transplantation, International Journal of Oncology, 2021,48(03):129-135.
[2] Translational Medicine Research (Series Title), Translational Research in Stroke (Book Title), Paul A. Lapchak and Guo yuan Yang (Eds) (ISBN: 978-981-10-5803-5). 2017年出版。(论著,本人参编)中风的转化研究。
[3] Gao S #*, Zhou F, Chen X, Xu J, human Adipose Derived Stem Cells Can Partially Rescue the Memory and the Spacial Learning of Stroke Mice,BIT 7th Annual World Congress of Regenerative Medicine & Stem Cells, Oral Presentation, 2014.11.13-2014.11.16. 会议论文,人脂肪干细胞能部分挽救中风小鼠的记忆和空间学习功能。
[4] Gao S#*, Wang J, Xu J, Transdifferentiation of human adipose-derived stem cells (hADSCs) into motoneuron-like cells for cell replacement therapy in spinal cord injury, BIT 8th Annual World Congress of Regenerative Medicine & Stem Cells, Oral Presentation, 2015.11.18-2015.11.20. 会议论文,人脂肪源性干细胞(hADSCs)转分化为运动神经元样细胞用于脊髓损伤的细胞替代治疗。
[5] Gao S#* Fengjuan Gao, XuChen, Jun Xu, Transdifferentiation of human adipose-derived stem cells (hADSCs) into neuron-like cells for cell replacement therapy in Middle Cerebral Artery Occlusion mouse model, Tissue Engineering & Regenerative Medicine International Society- Asia Pacific Meeting, Invited Oral Presentation, 2016.09.03-2016.09.06. 特邀会议报告,在大脑中动脉闭塞小鼠模型中,人脂肪来源干细胞(hADSCs)转分化为神经元样细胞用于细胞替代治疗。
[6] Attending International Society for Stem Cell Research (ISSCR), Guangzhou 2017.11.10- 2017.11.12.参加国际干细胞研究协会(ISSCR),广州,2017.11.10- 2017.11.12。
[7] Tai Wan Tsing Hua University Specially Invited Communication Report, “hADSC derived intermediate cell source for Parkinson Disease Treatment”. 2019.2.11 特邀交流报告 台湾清华大学特邀交流报告,人源脂肪干细胞转化而来的中间体细胞对帕金森的治疗,2019.2.11。
[8] East Hospital Craniocerebral Trauma and Neurology Intensive Forum, “human Adipose-Derived Stem Cells and Neural Regeneration”. 2020.12.04 特邀报告 东方医院颅脑创伤与神经病学集中论坛, 人源脂肪干细胞与神经再生,2020.12.04。
[9] 2021年应邀参加“International Conference on Neuro & Brain Disorders”作题为“Rebuilding of a Neuronal Circuit via Cell Replacement with hADSC-derived Neuron Cells for Treating Ischemic Stroke”专题汇报,2021.11.15。
2021年应邀参加“神经和大脑疾病国际会议”,作题为“hADSC衍生神经元细胞通过细胞替换重建神经回路治疗缺血性中风” 专题汇报,2021.11.15。 【科研项目】:
1. 国家科技部科技创新重点专项对台合作项目,2017YFE0121100、杆状病毒瞬时重编程人源脂肪干细胞制备临床级治疗帕金森疾病的种子细胞的机制研究、2018/06-2021/12、128万元、在研、主持。
The Ministry of Science and Technology Key Project, 2017YFE0121100, Non-integrated transient reprogramming hADSCs to produce clinical dopaminergic progenitor cells to treat Parkinson Disease and the mechanism, 06/2018-12/2021, ¥1280000, In research, host.
2. 上海市科委科技创新国际合作项目,18410760100、杆状病毒非整合瞬时过表达microRNA重编程人源脂肪干细胞为运动神经元细胞治疗脊髓损伤的研究、2018/06-2021/08、40万元、在研、主持。
Science and Technology Innovation International Cooperation project of Shanghai Science and Technology Commission, 18410760100, BCV transient overexpressing microRNA to reprogram hADSCs into motor neuron cells to treat spinal cord injury, 06/2018-08/2021, ¥400000, In research, host.
3. 国家自然科学基金青年项目,31601102、人源脂肪干细胞体外分化的运动神经元样细胞治疗小鼠脊髓损伤的机理研究、2017/01-2019/12、20万元、已结题、主持。
Youth Project of National Natural Science Foundation of China, 31601102, The mechanism study of hADSCs transdifferentiated into motor neuron like cells to treat spinal cord injury, 01/2017-12/2019, ¥200000, In research, host.
4. 上海市自然科学基金面上项目,16ZR1446900、网络建模解析人源脂肪干细胞多向分化的表观遗传命运决定、2016/07-2019/06、20万元、已结题、主持。
General project of Shanghai Natural Science Foundation, 16ZR1446900, Network Modelling the Epigenetic fate determination of hADSCs multi-differentiation, 07/2016-06/2019, ¥200000, Finished, host.
5. 重点项目培育类/国自然培育项目,22120170038、人源脂肪干细胞及分化的神经元样通过调节宿主神经细胞的线粒体自噬显著提高新生儿缺氧缺血小鼠的学习记忆能力、2017/09-2018/06、5万、已结题、主持。
Key Incubation project/NSFC Incubation project, 22120170038, HADSCs transdifferentiated into neuron-like cells to improve the learning and memory of HIE mouse by regulating the mitophagy of host neuron cells, 09/2017-06/2018, ¥50000, Finished, host.
6. 上海市卫生和健康委员会科研课题面上项目,201640056、人源脂肪干细胞分化的神经元样细胞治疗新生儿缺氧缺血脑病的研究、2017/01-2019/12、5万元、已结题、主持。
Shanghai Municipal Health commission scientific Research Project, 201640056, Treatment of neonatal hypoxic-ischemic encephalopathy with neuron-like cells differentiated from human adipocytes, 01/2017-12/2019, ¥50000, Finished, host.
7. 141-985工程重点建设项目,1500141834、人源脂肪干细胞治疗脑卒中的机理研究、2015/01-2015/06、5万元、已结题、主持。
141-985 Key Engineering Construction Project, 1500141834, The Mechanism study of hADSCs treating stroke, 01/2015-06/2015, ¥50000, Finished, host.
8. 国际合作项目,81461138037、在基于C9ORF72-ALS患者特异性iPS肌萎缩侧 索硬化疾病模型中研究G4C2重复扩增的致病机理、2015/01-2017/12、300万元、已结题、参加。
Key International Co-operation Project (NSFC), 81461138037, The pathogenesis study of Amyotrophic Lateral Sclerosis based on the G4C2 repeating with C9ORF72-ALS derived iPSC model, 01/2015-10/2017, ¥3000000, Finished, participant.
9. 科技部重大科学研究计划973,2011CB966200、组织干细胞在器官衰老与再生中的作用及调控机制研究、2011/01-2016/12、100万元、已结题、参加。
Ministry of Science and Technology Key Project,973,2011CB966200, Adult stem cells function and regulation mechanism in the tissue aging and regeneration, 01/2011-12/2016, ¥1000000, Finished, participant.
10. 科技部重大科学研究计划973,2010CB945200、基于诱导多能干细胞技术的 若干重大疾病模型与机理研究、2010/01-2015/12、275万元、已结题、参加。
Ministry of Science and Technology Key Project,973,2010CB945200, Study on several major disease models and mechanisms based on induced pluripotent stem cell technology, 01/2010-12/2015, ¥2750000, Finished, participant.
11. 高校青年教师培育人才计划,人源脂肪干细胞结合水凝胶材料治疗脑卒中的研究,2010/09-2012/09、10万元、已结题、主持。
Talent Cultivation Program for Young Teachers in Universities, Study on the treatment of stroke by combining human adipose stem cells with hydrogel materials, 09/2010-09/2012, ¥100000, Finished, host.