以miRNA敲除小鼠模型研究多基因参与的复杂慢性疾病的机制和药物，包括三个方面：

1） 感觉神经元发育和相关疾病（疼痛、听力损伤）的机制及药物研究。

2） 研究中脑多巴胺神经元（mDA）的存活机制，探寻帕金森病的早期诊断biomarker和诊断方法。

3） 神经与免疫及炎症性疼痛。

Research Interests

My lab is using miRNA knockout mouse models to study the mechanism and drug of complex chronic diseases involving multiple genes, including three directions:

1) Development of sensory neurons, and the mechanism and drug of related diseases (pain & hearing loss).

2) To study the survival mechanism of midbrain dopamine neurons, and to explore the early diagnosis biomarker and diagnostic methods of Parkinson's disease.

3) Neuroimmunology, Neuroinflammation & inflammatory pain.

2017.9-至今 特聘研究员、硕士研究生导师、博士研究生导师，同济大学医学院、转化医学高等研究院。

2012.6-2017.6 博士后研究员，瑞典卡罗林斯卡医学院医学生化与生物物理系。

2008.2- 2012.5 博士，德国国家环境与健康研究中心/慕尼黑工业大学，生物学。

2006.7-2007.10 研究助理，中科院上海生命科学研究院健康科学研究所。

Position

2017.9-Present Principal Investigator, Supervisor of doctoral and master's student, School of Medicine, Tongji University.

2012.6-2017.6 Postdoctoral researcher, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Sweden. (Prof. Patrik Ernfors Group)

2008.2- 2012.5 PhD，Technical University of Munich, Biology.

2006.7-2007.10 Research assistant，Institute of Health Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences.

科研业绩

本人研究神经发育、神经疾病和神经炎症，相关成果以第一作者或通讯作者发表在Science，Nature Immunology, Progress in Neurobiology, Frontiers in immunology, Frontiers in molecular Neuroscience, Development和Journal of neuroscience（2011, 2012）等杂志上。获国内发明专利7项和国际发明专利3项，另有5项国际和2项国内发明专利在审查中。

研究发现：

1）miR-183家簇系统性地调节神经病理性疼痛和躯体感觉神经元的分化（Science 2017， Development 2018）。

2) miR-96抑制电压门控钠离子通道网络和钙离子通道网络来抑制脊髓敏化的神经病理性疼痛，Nav1.7在脊髓神经元中的异位表达参与脊髓损伤诱导的神经病理性疼痛（Progress in Neurobiology 2021, Front. Mol. Neurosci. 2023）。

3）miR-146a和NPM1是炎症性肠病的潜在治疗靶点（Nature Immunology 2024, Front Immunol. 2024）。

Research Achievement

My research focuses on neurodevelopment, neurological diseases and neuroinflammation. The research findings have been published as the first author or corresponding author in Science, Nature Immunology, Progress in Neurobiology, Frontiers in immunology, Frontiers in molecular Neuroscience, Development and Journal of Neuroscience (2011, 2012). I have obtained 7 domestic patents and 3 international patents, and 7 patents are pending.

Recent findings:

1) miR-183 clusters systematically modulate neuropathic pain and somatosensory neuron differentiation (Science 2017, Development 2018).

2) miR-96 inhibits voltage-gated sodium channel network and calcium channel network to inhibit spinal cord sensitized neuropathic pain, and ectopic expression of Nav1.7 in spinal cord neurons is involved in spinal cord injury-induced neuropathic pain (Progress in Neurobiology 2021, Front. Mol. Neurosci. 2023).

3) miR-146a and NPM1 are potential therapeutic targets for inflammatory bowel disease (Nature Immunology 2024, Front Immunol. 2024).

发表的论文（Publications）

1. Zhao R, Yang J, Zhai Y, Zhang H, Zhou Y, Hong L, Yuan D, Xia R, Liu Y, Pan J, Shafi S, Shi G, Zhang R, Luo D, Yuan J, Pan D, Peng C*, Li S*, Sun M*. Nucleophosmin 1 promotes mucosal immunity by supporting mitochondrial oxidative phosphorylation and ILC3 activity. Nat Immunol. 2024 Aug 5. doi: 10.1038/s41590-024-01921-x. （*Corresponding author）

2. Zhu F, Yang T, Ning M, Liu Y, Xia W, Fu Y, Wen T, Zheng M, Xia R, Qian R, Li Y, Sun M, Liu J, Tian L*, Zhou Q*, Yu X*, Peng C*. MiR-146a alleviates inflammatory bowel disease in mice through systematic regulation of multiple genetic networks. Front Immunol. 2024 May 10;15:1366319. doi: 10.3389/fimmu.2024.1366319. PMID: 38799464; PMCID: PMC11116640. (*Corresponding author)

3. Yan Fu, Liting Sun, Fengting Zhu, Wei Xia, Ting Wen, Ruilong Xia, Xin Yu, Dan Xu*, Changgeng Peng*. Ectopic expression of Nav1.7 in spinal dorsal horn neurons induced by NGF contributed to neuropathic pain in a mouse spinal cord injury model. Front. Mol. Neurosci., 03 March 2023. Volume 16 – 2023. (*Corresponding author)

4. Liting Sun, Hang Xian, Yunxin Shi, Taotan Yang, Hongyan Shuai, Ruilong Xia, Ting Wen, Wei Xia, Ran Qian, Fengting Zhu, Yuanying Liu, Zhicheng Tian, Lamei Li, Rui Cong, Ceng Luo, Shengxi Wu, Xiafeng Shen*, Xin Yu*, Rou-Gang Xie*, Changgeng Peng*. Nav1.7 and Nav1.8 form supramolecular active clusters with TRKB in mouse and human DRG neurons during development of neuropathic pain. medRxiv 2022.11.05.22281929; doi: https://doi.org/10.1101/2022.11.05.22281929 (*Corresponding author)

5. Liting Sun, Changgeng Peng, Elbert Joosten, Chi Wai Cheung, Fei Tan, Wencheng Jiang, and Xiafeng Shen. Spinal Cord Stimulation and Treatment of Peripheral or Central Neuropathic Pain: Mechanisms and Clinical Application. Neural Plast. 2021; 2021: 5607898.

6. Yong-Hui Zhang, Hao-Yu Hu, Yuan-Chang Xiong, Changgeng Peng, Li Hu, Ya-Zhuo Kong, Yu-Ling Wang, Jia-Bao Guo, Sheng Bi, Tie-Shan Li, Li-Juan Ao, Chu-Huai Wang, Yu-Long Bai, Lei Fang, Chao Ma, Lin-Rong Liao, Hao Liu, Yi Zhu, Zhi-Jie Zhang, Chun-Long Liu, Guo-En Fang, Xue-Qiang Wang. Exercise for Neuropathic Pain: A Systematic Review and Expert Consensus. Front Med (Lausanne). 2021 Nov 24;8:756940.

7. Sun L, Xia R, Jiang J, Wen T, Huang Z, Qian R, Zhang M, Zhou M, Peng C. MicroRNA-96 is required to prevent allodynia by repressing voltage-gated sodium channels in spinal cord. Progress in Neurobiology, 2021 Jul;202:102024.

8. Li N, Zhou P, Tang H, He L, Fang X, Zhao J, Wang X, Qi Y, Sun C, Lin Y, Qin F, Yang M, Zhang Z, Liao C, Zheng S, Peng X, Xue T, Zhu Q, Li H, Li Y, Liu L, Huang J, Liu L, Peng C, Kaindl AM, Gecz J, Han D, Liu D, Xu K, Hu H. In-depth analysis reveals complex molecular aetiology in a cohort of idiopathic cerebral palsy. Brain. 2021 Jun 2: doi: 10.1093/brain/awab209.

9. Zhou P, Qi Y, Fang X, Yang M, Zheng S, Liao C, Qin F, Liu L, Li H, Li Y, Ravindran E, Sun C, Wei X, Wang W, Fang L, Han D, Peng C, Chen W, Li N, Kaindl AM, Hu H. Arhgef2 regulates neural differentiation in the cerebral cortex through mRNA m6A-methylation of Npdc1 and Cend1. iScience. 2021 May 24;24(6):102645.

10. Nouri P, Goetz S, Rauser B, Irmler M, Peng C, Trumbach D, Kempny C, Lechermeier C, Brybiok A, Dulugos A, Euchner E, Beckers J, Brodski C, Klumper C, Wurst W, Prakash N. Dose-dependent and subset-specific regulation of midbrain dopaminergic neuron differentiation by LEF1-mediated WNT1/b-catenin signaling. Frontiers in Cell and Developmental Biology. 2020 Oct 26;8:587778.

11. Sun L#, Liu Y# and Peng C. Different Manners of Interplay between MicroRNAs and Gene Programs in Neuronal Specification. Journal of Cell Signaling. 2018, 3:4. DOI: 10.4172/2576-1471.1000193.

12. Peng C§，Furlan A, Zhang M, Su J, Lübke M， Lönnerberg P, Abdo H, Sontheimer J, Sundström E, Ernfors P2§. Termination of cell-type specification gene programs by miR-183 cluster determines the population sizes of low threshold mechanosensitive neurons. Development. 2018 Sep 25;145(18):dev165613. (§Corresponding author).

13. Zhang M, Su J, Adori C, Cinquina V, Malenczyk K, Girach F, Peng C, Ernfors P, Löw P, Borgius L, O, Watanabe M, M, Mitsios N, Mulder J, Harkany T*，Hökfelt T*. Ca2+-binding protein NECAB2 facilitates inflammatory pain hypersensitivity. Journal Of Clinical Investigation. 2018 July 30. https://doi.org/10.1172/JCI120913.

14. Yang S, Toledo E, Rosmaninho P, Peng C, Uhlén P, Castro D, Arenas E. A Zeb2-miR-200c loop controls midbrain dopaminergic neuron neurogenesis and migration. Communications Biology volume 1, Article number: 75 (2018).

15. Peng C, Li L, Zhang M, Gonzales C, Parisien M, Belfer I, Usoskin D, Abdo H, Furlan A, Häring M, Lallemend F, Harkany T, Diatchenko L, Hökfelt T, Hjerling-Leffler J and Ernfors P. MiR-183 cluster scales mechanical pain sensitivity by regulating basal and neuropathic pain genes. Science. 2017 Jun 16;356(6343):1168-1171. （Science同期杂志上发表了Perspective review， F1000优秀论文）

16. Peng C, Li N, Ng YK, Zhang J, Meier F, Theis FJ, Merkenschlager M, Chen W, Wurst W, Prakash N. A unilateral negative feedback loop between miR-200 microRNAs and Sox2/E2F3 controls neural progenitor cell-cycle exit and differentiation. J Neurosci. 2012 Sep 19;32(38):13292-308. （F1000优秀论文）

17. Peng C, Aron L, Klein R, Li M, Wurst W, Prakash N, and Le W. Pitx3 is a critical mediator of GDNF-induced BDNF expression in nigrostriatal dopaminergic neurons. J Neurosci. 2011 Sep;31, 12802-12815.

18. Peng C#, Fan S#, Li X, Fan X, Ming M, Sun Z, Le W. Overexpression of pitx3 upregulates expression of BDNF and GDNF in SH-SY5Y cells and primary ventral mesencephalic cultures. FEBS Lett. 2007 Apr 3;581(7):1357-61. Epub 2007 Mar 2.

19. Li X, Peng C, Li L, Ming M, Yang D, Le W. Glial cell-derived neurotrophic factor protects against proteasome inhibition-induced dopamine neuron degeneration by suppression of endoplasmic reticulum stress and caspase-3 activation. J Gerontol A Biol Sci Med Sci. 2007 Sep;62(9):943-50.

20. Yang, D., Peng, C., Li, X., Fan, X., Li, L., Ming, M., Chen, S., and Le, W. Pitx3-transfected astrocytes secrete brain-derived neurotrophic factor and glial cell line-derived neurotrophic factor and protect dopamine neurons in mesencephalon cultures. J Neurosci Res.2008 Nov; 86(15), 3393-3400.

21. Li X, Yang D, Li L, Peng C, Chen S, Le W. Proteasome inhibitor lactacystin disturbs the intracellular calcium homeostasis of dopamine neurons in ventral mesencephalic cultures. Neurochem Int. 2007 Jun;50(7-8):959-65. Epub 2007 May 3.

授权和申请的发明专利 （Patents）

1. ANALGESIC AND ANTIPRURITIC PHARMACEUTICAL COMPOSITION AND APPLICATION METHOD THEREFOR.2021329457, Inverntor：Changgeng Peng，Liting Sun，Ruilong Xia. Australia. 2023.

2. ANALGESIC AND ANTIPRURITIC PHARMACEUTICAL COMPOSITION AND APPLICATION METHOD THEREFOR. 18/171，431, Inverntor：Changgeng Peng，Liting Sun，Ruilong Xia. American. 2024.

3. 《一种止痛止痒药物组合物及其应用方法》，专利号：ZL202010845095.2，发明人设计人：彭长庚，孙立婷，夏瑞龙。

4. 《一种制备TrkB-Tomato小鼠的方法及其应用》专利申请号：202210052495.7，发明人设计人：彭长庚，解柔刚，孙立婷，夏瑞龙。

5.《Pitx3基因及其启动子的应用》，专利号：ZL200610025416.4，发明人设计人：乐卫东，彭长庚。

主持项目：

1. 国家自然科学基金委，面上项目，32070977，调控中脑黑质多巴胺神经元诞生数目的分子机制研究，2021.01-2024.12，主持。

2. 国家自然科学基金委，面上项目，51971236，钛植入体对骨免疫和神经再生微环境的光致调控机制和效果研究，2020.01-2023.12，二级主持,已完成。

3. 国家自然科学基金委，面上项目，31871063，MiR-183-96在中枢神经系统中调制急性和神经病理性疼痛的作用及机制研究，2019.01-2020.12，主持,已完成。

4. 国家自然科学基金委，应急管理项目，31741057，利用基因敲除小鼠优选治疗神经病理性疼痛的候选微RNA靶点，2018.01-2018.12主持,已完成。

5. 国家自然科学基金委，青年项目，82101320，髓背角神经元中Nav1.7在长期神经病理性痛中的贡献研究及靶性药筛模型构建，2022.01.2024.12。（课题组前成员孙立婷博士主持）

Grants：

1. National Natural Science Foundation of China, General Project, 32070977, Molecular Mechanism Regulating the Number of Dopamine Neurons in the Substantia Nigra in the Midbrain, 2021.01-2024.12, Principal Investigator.

2. National Natural Science Foundation of China, General Project, 51971236, Research on the Photoregulatory Mechanism and Effect of Titanium Implants on Bone Immunity and Nerve Regeneration Microenvironment, 2020.01-2023.12, Principal Investigator, Completed.

3. National Natural Science Foundation of China, 31871063, Study on the role and mechanism of MiR-183-96 in modulating acute and neuropathic pain in the central nervous system, 2019.01-2020.12, Principal Investigator, completed.

4. National Natural Science Foundation of China, Emergency Management Project, 31741057, Candidate MicroRNA Targets for the Treatment of Neuropathic Pain Using Knockout Mice, 2018.01-2018.12, Completed.

5. National Natural Science Foundation of China, Youth Program, 82101320, Research on the contribution of Nav1.7 in long-term neuropathic pain in medullary dorsal horn neurons and construction of targeted drug screening model, 2022.01.2024.12. (Chaired by Dr. Sun Liting, former member of Lab)

获奖

1. 《镇痛止痒1.1类小分子药物研发》，2023年中国发明协会银奖，证书号：3102117。第一完成人。

2. 同济大学2021年十大最具转化潜力科技成果奖。

3. 《中脑多巴胺系统发育及神经元变性在帕金森病中的基因调控和分子机制》2008年中华医学科技奖二等奖，证书号：200802106P1009。

Awards

1. "R&D of Small Molecule Drugs for Analgesic and Itch Relief", 2023 China Association of Inventions Silver Award, certificate number: 3102117. The first finisher.

2. Tongji University 2021 Top Ten Most Promising Scientific and Technological Achievement Award.

3. "Gene Regulation and Molecular Mechanism of Dopamine Phylogeny and Neuronal Degeneration in Parkinson's Disease", 2008 Chinese Medical Science and Technology Award, Certificate No. 200802106P1009.