毕业于华中科技大学同济医学院，英国剑桥大学访问学者。主持国家自然基金等项目20余项。以通讯作者在NAR、Science advance、EMBO J和JECCR等高水平期刊发表SCI论文50余篇。授权发明专利2项。获上海市医学科技奖1项。撰写论著2部。获上海市医学科技奖1项《子宫颈肿瘤的基础研究和创新性手术治疗体系》。
Prof. Fang Li is a graduate of Tongji Medical College of Huazhong University of Science and Technology and is engaged in research at the University of Cambridge in the UK as a visiting scholar. She has overseen the implementation of over 20 projects funded by the National Natural Science Foundation of China (NSFC) and other organizations. She has published over 50 papers in high-impact journals, including Nucleic Acid Research, Science Advances, EMBO Journal, and Journal of Experimental & Clinical Research, acting as the corresponding author or first author on these publications. She has been granted two invention patents, and has also authored two books. She was the recipient of the Shanghai Medical Science and Technology Award for her contributions to “the development of a novel approach to the treatment of uterine cervical tumors with combination on fundamental research and innovative surgical techniques”.
主持基金；
foundation projects:
国家自然科学基金面上项目（批准号：82273091），“HPV58与人融合基因E1-C通过激活PI3K/Akt通路诱导宫颈癌发生的机制研究”，2023/01 - 2026/12，52万元，在研，项目负责人。 国家重点研发计划“生育健康及妇女儿童健康保障”专项（批准号：2021YFC2701200），“宫颈病变的精准筛查和防治研究”项目课题5“靶向阻遏 HPV 持续性感染恶性转化的治疗策略研究”，2022/01-2024/12，40万，在研，课题骨干。
上海市卫健委科研项目（批准号：202150001），“高危 HPV mRNA 特异序列检测和显微内镜在体病理分流诊断宫颈癌前病变”，2022/01-2024/12，20万元，在研，项目负责人。
上海市科委课题（批准号：21S31905100），“II类医疗器械显微内镜人工智能病理分析系统样机的研发”， 2021/07/01-2024/06/30，25万元，在研，项目负责人。
同济大学研究生教材建设项目（批准号：2020JC21），“显微内镜诊断宫颈病变”，2020/01-2021/12，已结题，项目负责人。
同济大学“中央高校基本科研业务费”专项资金项目（批准号：22120190241），“显微内镜实时在体诊断消化道和子宫颈癌前病变”,2019/10 - 2020/11，20万元，已结题，项目负责人。
同济大学“中央高校基本科研业务费”专项资金项目（批准号：22120190214），“智能型显微内镜及其在宫颈癌筛查诊断中的应用研究”,2019/08 - 2021/07，60万元，已结题，项目负责人。
国家自然科学基金面上项目（批准号：81771529），“HPV16整合所致宿主顺式元件变异在子宫颈上皮内瘤变进展中的作用及分子机制研究”,2018/01 - 2021/12，55万元，已结题，项目负责人。
国家科学技术学术著作出版基金，2017-H-047,阴道内镜学：基础与临床，2017，已结题，项目负责人。
上海市科委课题（批准号：17441902500），“全景内置显微阴道镜诊断子宫颈病变”，2017/06-2020/09，40万元，已结题，项目负责人。
上海市申康课题（批准号：16CR3089B），“体内显微阴道镜技术在子宫颈癌诊断中的应用”，2016/12-2019/06。30万元，已结题，项目负责人。
上海市科委课题（批准号：16411950200），“宫颈癌早诊优治体系建立研究”，2016/10-2019/09，200万元，已结题，参加。
上海市科委课题（批准号：15411967700），“检测染色体特征性改变诊断子宫颈上皮内瘤变”，2015/10-2018/09，10万元，已结题，项目负责人。
上海市科委课题（批准号：124119a3800），“检测高危型HPV-DNA整合状态预测子宫颈癌变”，2012/10-2015/09，20万元，已结题，项目负责人。
上海市申康课题（批准号：SHDC12012315)，“妇女两癌（乳腺癌、宫颈癌）综合防治模式研究”, 2013/01-2015/12，10万元，已结题，子宫颈癌筛查小组负责人 。
上海市卫生局课题（批准号：20114150），“子宫颈高危HPV感染患者HPV E5蛋白和E2检测及在癌前病变转化中的作用”，2012/01-2014/12, 6万元，已结题，项目负责人。
上海市科委课题（批准号：1052nm01200）： “磁性纳米载体介导的PlGF受体靶向阻断抑制卵巢癌血管生成的研究”2010/01-2012/12, 20万元。已结题。项目负责人。
国家自然科学基金课题(批准号： 30672232) “免疫磁性纳米微球用于卵巢癌靶向治疗和体内成像的探索性研究” 2007/01-2009/12，30万元，已结题，项目负责人。
上海市科委课题（批准号：074119647）：“量子点标记的特异性肽用于卵巢癌早期诊断的研究”2007/01-2010/12，20 万元 已结题。项目负责人。
上海市卫生局课题（批准号：2007147）：“纳米缓释药腹腔内灌注治疗卵巢癌的研究”2007/01-2010/12，3万元 已结题。项目负责人。
代表性研究成果：
Representative research publications:
Guangxu Cao#, Jiali Yue#, Yetian Ruan#, Ya Han, Yong Zhi, Jianqiao Lu, Min Liu, Xinxin Xu, Jin Wang, Quan Gu, Xuejun Wen, Jinli Gao, Qingfeng Zhang, Jiuhong Kang, Chenfei Wang*, Fang Li*. Single-cell Dissection of Cervical Cancer Reveals Key Subsets of the Tumor Immune Microenvironment. EMBO JOURNAL. 2023 Aug 15;42(16):e110757. IF=11.4
Hu B#, Wang R#, Wu D, Long R, Fan J, Hu Z, Hu X, Ma D, Li F*, Sun C*, Liao S*. A Promising New Model: Establishment of Patient-Derived Organoid Models Covering HPV-Related Cervical Pre-Cancerous Lesions and Their Cancers. Adv Sci (Weinh). 2024 Mar;11(12):e2302340. IF=15.1
Xiao Y#, Zhi Y, Cao G, Ma H, Gao J*, Li F*. Primary Ewing’s sarcoma of the uterine cervix: a case report and review of the literature. J Cancer Res Clin Oncol. 2024 May 21;150(5):267. doi: 10.1007/s00432-024-05698-2. IF=2.7
Liu M#, Han Z#, Zhi Y, Ruan Y, Cao G, Wang G, Xu X, Mu J, Kang J, Dai F, Wen X, Zhang Q*, Li F*. Long-read sequencing reveals oncogenic mechanism of HPV-human fusion transcripts in cervical cancer. Transl Res. 2023 Mar;253:80-94. doi: 10.1016/j.trsl.. IF=10.17
Ruan Y#, Li H#, Liu M, Cao G, Xu X, Han L, Li F*. A retrospective analysis of human papillomavirus (HPV) prevalence and genotype distribution among 25,238 women in Shanghai, China revealed the limitations of current HPV-based screening and HPV vaccine. Cancer Epidemiol. 2023 Jun;84:102372. doi: 10.1016/j.canep.2023.102372. Epub 2023 Apr 27. PMID: 37119603. IF=2.6
Liu M#, Lu J#, Zhi Y, Ruan Y, Cao G, Xu X, An X, Gao J, Li F*. Microendoscopy in vivo for the pathological diagnosis of cervical precancerous lesions and early cervical cancer. Infect Agent Cancer. 2023 Apr 26;18(1):26. doi: 10.1186/s13027-023-00498-8. PMID: 37101242; PMCID: PMC10134531. IF=3.7
Lei L#, Xu X, Gong C, Lin B*, Li F*. Integrated analysis of genome-wide gene expression and DNA methylation profiles reveals candidate genes in ovary endometriosis. Front Endocrinol (Lausanne). 2023 Mar 23;14:1093683. doi: 10.3389/fendo.2023.1093683. PMID: 37033258; PMCID: PMC10076879. IF=5.2
Zhi Y#, Chen F, Cao G, Li F*. Gamma-Irradiated Non-Capsule Group B Streptococcus Promotes T-Cell Dependent Immunity and Provides a Cross-Protective Reaction. Pharmaceuticals (Basel). 2023 Feb 20;16(2):321. doi: 10.3390/ph16020321. PMID: 37259463; PMCID: PMC9961543. IF=4.6
Zhi Y#, Chen X, Cao G, Chen F, Seo HS, Li F*. The effects of air pollutants exposure on the transmission and severity of invasive infection caused by an opportunistic pathogen Streptococcus pyogenes. Environ Pollut. 2022 Oct 1;310:119826. IF=9.98
Hu H#, Yang M#, Dong W#, Yin B, Ding J, Huang B, Zheng Q*, Li F*, Han L*. A Pyroptosis-Related Gene Panel for Predicting the Prognosis and Immune Microenvironment of Cervical Cancer. Front Oncol. 2022 Apr 29;12:873725. IF=5.7
Jianyi D#, Haili G#, Bo Y#, Meiqin Y, Baoyou H, Haoran H, Fang L*, Qingliang Z*, Lingfei H*. Myeloid-derived suppressor cells cross-talk with B10 cells by BAFF/BAFF-R pathway to promote immunosuppression in cervical cancer. Cancer Immunol Immunother. 2022 Jun 20. IF=6.3
Yang, M.#, Hu, H.#, Wu, S., Ding, J., Yin, B., Huang, B., Li, F.*, Guo, X.*, & Han, L*. (2022). EIF4A3-regulated circ_0087429 can reverse EMT and inhibit the progression of cervical cancer via miR-5003-3p-dependent upregulation of OGN expression. Journal of experimental & clinical cancer research : CR, 41(1), 165. IF=12.66
Yin Bo#, Ding Jianyi#, Hu Haoran#, Yang Meiqin, Huang Baoyou, Dong Wei*, Li Fang*, Han Lingfei*. Overexpressed CMTM6 improves prognosis and associated with immune infiltrates of ovarian cancer. Front Mol Biosci.2021. IF=6.11
Le Fu#, Wei Xia#, Wei Shi, Guangxu Cao, Yetian Ruana, Xingyu Zhao, Min Liu, Sumei Niu, Fang Li*, Xin Gao*. Deep learning based cervical screening by the cross-modal integration of colposcopy, cytology, and HPV test. International Journal of Medical Informatics,159 (2022) 104675. IF=4.73
Xinxin Xu#, Zhiqiang Han#, Yetian Ruan, Min Liu, Guangxu Cao, Chao Li*, Fang Li*. HPV16-LINC00393 integration alters local 3D genome architecture in cervical cancer cells. Front Cell Infect Microbiol. 2021 Dec 7;11:785169. IF=6.07
Zhiyuan Huang#, Fang Li*, Qinchuan Li*. Expression profile of RNA binding protein in cervical cancer using bioinformatics approach. Cancer Cell Int. 2021 Dec 4;21(1):647. IF=6.42
Li Y#, Gong YX#, Wang Q, Gao S, Zhang H, Xie F, Cong Q, Chen L, Zhou Q, Hong Z, Qiu L, Li F, Xie Y*, Sui L*. Optimizing the Detection of Occult Cervical Cancer: A Prospective Multicentre Study in China. Int J Womens Health. 2021 Oct 27;13:1005-1015. IF=2.59
Zhu J#, Chen F, Luo L, Wu W, Dai J, Zhong J, Lin X, Chai C, Ding P, Liang L, Wang S, Ding X, Chen Y, Wang H, Qiu J, Wang F, Sun C, Zeng Y, Fang J, Jiang X, Liu P, Tang G, Qiu X, Zhang X, Ruan Y, Jiang S, Li J, Zhu S, Xu X, Li F, Liu Z, Cao G*, Chen D*. Single-cell atlas of domestic pig cerebral cortex and hypothalamus. Science Bulletin 2021, 66(14): 1448-1461. IF=20.57
Ruan Y#, Liu M, Guo J, Zhao J, Niu S, Li Fang*, Evaluation of the accuracy of colposcopy in detecting high-grade squamous intraepithelial lesion and cervical cancer. Arch Gynecol Obstet. 2020 Aug;10.1007/s00404-020-05740-x. doi:10.1007/s00404-020-05740-x IF=2.49
Guo, Junhan#, Fu Le#, Zhao Junwei, Lei Lei, Zhan Qin, Liu Min, Ruan Yetian, Li Hui, Xu Jin, Li Nana, Wang Hanlin, Zhu Huiting, Han Zhiqiang, Li Fang*, The value of microendoscopy in the diagnosis of cervical precancerous lesions and cervical microinvasive carcinoma. Archives of Gynecology and Obstetrics. 2020 June 302. doi:10.1007/s00404-020-05565-8. IF=2.49
Wen Yu#, Cai Long, Tailin Zhu, Huiting Zhu, Zhiqiang Han*, Fang Li*, High resolution multispectral endoscopy significantly improves the diagnostic accuracy of cervical intraepithelial lesions. Journal of obstetrics and gynaecology research, 2020 April, 46: 939-944. doi:10.1111/jog.14241. IF=1.69
Chao Li#*, Hongfeng Ao#, Guofang Chen, Fang Wang, Fang Li*. The Interaction of CDH20 With β-Catenin Inhibits Cervical Cancer Cell Migration and Invasion via TGF-β/Smad/SNAIL Mediated EMT. Front Oncol. 2020 Jan 9;9:1481. doi: 10.3389/fonc.2019.01481. IF=5.7
Junwei Zhao#, Qin Zhan, Junhan Guo, Min Liu, Yetian Ruan, Tailin Zhu, Lingfei Han*, Fang Li*. Phylogeny and polymorphism in the E6 and E7 of human papillomavirus: alpha-9 (HPV16, 31, 33, 52, 58), alpha-5 (HPV51), alpha-6 (HPV53, 66), alpha-7 (HPV18, 39, 59, 68) and alpha-10 (HPV6, 44) in women from Shanghai. Infect Agent Cancer. 2019 Nov 21;14:38. doi: 10.1186/s13027-019-0250-9. IF=3.69
Junwei Zhao#, Jiacheng Zhu, Junhan Guo, Tailin Zhu, Jixing Zhong, Min Liu, Yetian Ruan, Shujie Liao*, Fang Li*. Genetic variability and functional implication of HPV16 from cervical intraepithelial neoplasia in Shanghai women. J Med Virol. 2020 Mar;92(3):372-381. doi: 10.1002/jmv.25618. IF=20.69
Chao Li#*, Yanfei Li#, Lanxia Sui, Jian Wang, Fang Li*, Phenyllactic acid promotes cell migration and invasion in cervical cancer via IKK/NF-κB-mediated MMP-9 activation. Cancer Cell Int. 2019 Sep 23;19:241. doi: 10.1186/s12935-019-0965-0. IF=6.42
Huiyan Hu#, Jingjing Zhao#, Wen Yu, Junwei Zhao, Zhewei Wang, Lin Jin, Yunyun Yu, Lingfei Han, Lu Wang, Huiting Zhu*, Fang Li*, Human papillomavirus DNA, HPV L1 capsid protein and p16INK4a protein as markers to predict cervical lesion progression. Archives of Gynecology and Obstetrics. 2019 Jan, 299(1):141-149. IF=2.49
Junwei Zhao#, Lu Wang#, Hui Lin, Wen Yu, Xianghong Xu, Huiyan Hu, Laifang Zhu, Lingfei Han*, Fang Li*, Association of HLA-DRB1/DQB1 polymorphism with high-risk HPV infection and cervical intraepithelial neoplasia women from Shanghai. International Journal of Clinical and Experimental Pathology, 2018.2.15, 11(2):748-756.
Yahui Zhang#, Lin Wang#, Xiang Xu#, Fang Li*, Qingsheng Wu*, Combined systems of different antibiotics with nano-CuO against Escherichia coli and the mechanisms involved. Nanomedicine (Lond), 2018 Feb; 13(3):339-351. IF=6.09
Xiaojie Huang#, Chao Li#, Fang Li, Junwei Zhao, Xiaoping Wan*, Kai Wang*, Cervicovaginal microbiota composition correlates with the acquisition of high- risk human papillomavirus types. International Journal of Cancer, 2018 Aug 1, 143(3):621-634. IF=7.31
Dongsheng Chen#*, Sanjie Jiang, Xiaoyan Ma, Fang Li*, TFBSbank: a platform to dissect the big data of protein-DNA interaction in human and model species. Nucleic Acids Research, 2017.1.4, 45(D1): D151-D157. IF=19.6
Xiaohui Zhou#*, Yanfeng Shi, Lijie Wang, Mei Liu, Fang Li*, Distribution Characteristics of Human Papillomavirus Infection: A Study Based on Data from Physical Examination. Asian Pacific Journal of Cancer Prevention, 2017 Jul 27, 18(7):1875-1879.
Junwei Zhao#, Fang Fang#, Yi Guo, Tailin Zhu, Yunyun Yu, Fanfei Kong, Lingfei Han, Dongsheng Chen*, Fang Li*, HPV16 integration probably contributes to cervical oncogenesis through interrupting tumor suppressor genes and inducing chromosome instability. Journal of Experimental & Clinical Cancer Research, 2016.11.25, 35(1): 180-180. IF=12.66
Yunyun Yu#, Lingfei Han#, Wen Yu, Yankang Duan, Zhewei Wang, Huiyan Hu, Junwei Zhao, Suman Singh, Fanfei Kong, Lin Jin, Jing Sun*, Fang Li*, High risk factors associated with HPV persistence after loop electrosurgical excision procedure in patients with intraepithelial neoplasia. International Journal of Clinical and Experimental Pathology, 2017,10(2):1817-1824.
Yi Guo#, Jiachang Hu, Liping Zhu, Jing Sun, Longxu Xie, Fanfei Kong, Lingfei Han, Fang Li*, Physical Status and Variant Analysis of Human Papillomavirus 16 in Women from Shanghai. Gynecologic and Obstetric Investigation, 2016.01.01, 81(1):61-70. IF=2.72
Suman Singh#, Qian Zhou#, Yunyun Yu, Xianghong Xu, Xiaojie Huang, Junwei Zhao, Lingfei Han, Kai Wang, Jing Sun*, Fang Li*, Distribution of HPV genotypes in Shanghai women. International Journal of Clinical and Experimental Pathology, 2015.01.01, 8(9): 11901-11908.
Lingfei Han#*, Wei Wang#, Jiahong Lu#, Fanfei Kong, Ge Ma, Yiping Zhu, Dong Zhao, Jianlong Zhu, Wen Shuai, Qian Zhou, Ping Chen, Lei Ye, Jie Tao, Sarfraz Ahmad, Fang Li*, Jing Sun*, AAV-sBTLA facilitates HSP70 vaccine-triggered prophylactic antitumor immunity against a murine melanoma pulmonary metastasis model in vivo. Cancer Letters, 2014.11.28, 354(2):398-406. IF=9.75
Fang Li#*, Yi Guo, Lingfei Han*, Yankang Duan, Fang Fang, Sumei Niu, Qiujie Ba, Huaishi Zhu, Fanfei Kong, Chao Lin, Xuejun Wen, In vitro and in vivo growth inhibition of drug-resistant ovarian carcinoma cells using a combination of cisplatin and a TRAIL-encoding retrovirus. Oncology Letters, 2012.12.01, 4(6): 1254-1258. IF=3.11
Fang Li#*, Qiujie Ba#, Sumei Niu, Yi Guo, Yankang Duan, Peng Zhao, Chao Lin*, Jing Sun*, In-situ forming biodegradable glycol chitosan-based hydrogels: Synthesis, characterization, and chondrocyte culture. Materials Science and Engineering: C, 2012.10.1, 32(7): 2017-2025. IF=8.45
Fang Li#*, Jing Sun, Huaishi Zhu, Xuejun Wen, Chao Lin, Donglu Shi, Preparation and characterization novel polymer-coated magnetic nanoparticles as carriers for doxorubicin. Colloids and Surfaces B: Biointerfaces, 2011.11.1, 88(1): 58-62. IF=5.99
Xu Song#*, Fang Li#, Jingwei Ma, Nengqin Jia*, Jianming Xu, Hebai Shen*, Synthesis of fluorescent silica nanoparticles and their applications as fluorescence probes. Journal of Fluorescence, 2011.5.01, 21(3):1205-1212. IF=2.52
Fang Li#*, Sumei Niu, Jing Sun, Huaishi Zhu, Qiujie Ba, Yi Guo, Donglu Shi, Efficient In Vitro TRAIL-Gene Delivery in Drug-Resistant A2780/DDP Ovarian Cancer Cell Line via Magnetofection. Journal of Nanomaterials, 2011.01.01. IF=3.79
Fang Li#*, Pingping Su#, Chao Lin, Hong Li, Jiajing Cheng, Donglu Shi, Ribosome display and selection of human anti-placental growth factor scFv derived from ovarian cancer patients. Protein and Peptide Letters, 2010.5.01, 17(5):585-590. IF=1.92
Lin, Chao#*, Peng Zhao#, Fang Li*, Fangfang Guo, Zhuoquan Li, Xuejun Wen, Thermosensitive in situ-forming dextran-pluronic hydrogels through Michael addition. Materials Science and Engineering: C, 2010.01.01, 30(8):1236-1244. IF=8.45
Fang Li#*, Jianing Li, Xuejun Wen*, Shenghu Zhou, Xiaowen Tong, Pingping Su, Hong Li, Donglu Shi*, Anti-tumor activity of paclitaxel-loaded chitosan nanoparticles: An in vitro study. Materials Science and Engineering: C, 2009.01.01, 29(8):2392-2397. IF=8.45
Fang Li#, Li Meng#, Hui Xing, Jianfeng Zhou, Shixuan Wang, Lei Huang, Gang Xu, Huaishi Zhu, Yunping Lu, Ding Ma*, Essential role of c-Jun-NH2-terminal kinase on synergy induction of apoptosis by TRAIL plus ADM in ADM resistant MCF-7/ADM cells. Apoptosis, 2006.01.01, 11:1239-1246. IF=5.99
Fang Li#, Li Meng#, Jianfeng Zhou, Hui Xing, Shixuan Wang, Gang Xu, Huaishi Zhu,Beibei Wang, Gang Chen, Yunping Lu, Ding Ma*, Reversing chemoresistance in cisplatin-resistant human ovarian cancer cells: A role of c-Jun NH(2)-terminal kinase 1. Biochemical and Biophysical Research Communications, 2005.10.01, 335(4):1070-1077. IF=3.32
Lei Huang#, Qilin Ao, Qinghua Zhang, Xiaokui Yang, Hui Xing, Fang Li, Gang Chen, Jianfeng Zhou, Shixuan Wang, Gang Xu, Li Meng, YunPing Lu, Ding Ma*, Hypoxia induced paclitaxel resistance in human ovarian cancers via hypoxia-inducible factor 1 alpha. Journal of Cancer Research and Clinical Oncology, 2010.3.01, 136(3):447-456. IF=4.32
胡家昌(#)，李芳，高危型HPV DNA与宿主基因整合及致癌机理的相关研究进展，现代妇产科进展，2015.5.01，（05）：384~386
方芳，李芳，段燕康，宫颈病变患者人乳头瘤病毒感染分型研究，中国药房，2013.01.01，（34）：3241~3243
韩凌斐，郭晓青，胡家昌，孔繁飞，何拉曼，李芳，朱建龙，孙静，宫颈癌患者外周血髓源性抑制细胞的比例及其临床意义，华中科技大学学报(医学版)，2015.01.01，（01）：32~36
发明专利:
Patents：
李芳 皇甫江涛，一种内置式显微阴道镜，2018.06.01，ZL201710171035.5，发明专利。
陈惟,李芳,李锋华,李建中. 一种磁共振宫颈线圈[P]. 广东省：CN111035388A,2020-04-21.
获奖情况；
Awards:
子宫颈肿瘤的基础研究和创新性手术治疗体系, 上海市医学会, 上海市医学科技奖, 省部二等奖, 2021（刘晓军; 李芳; 金志军; 李超; 张志刚; 刘敏; 高京海）
学术会议报告:
Reports:
Fang Li, Identification HPV Integration Sites of CIN and Cervical Cancer Patients in Shanghai, EUROGIN 2016 Congress, Salzburg, Austria, June 15-18, 2016.
多次参加国家级学术会议并做报告。
主办《宫颈癌诊断治疗新进展》和《子宫颈疾病新进展》继续教育项目10届。
